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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene is observed in lots of tumors, which results in chromosome instability, and therefore could add to tumorigenesis. This protein was found to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a important role in advertising mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be described. Two further splice variants have been described but detailed nucleotide sequence data just isn't however out there.

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Blocking peptides are peptides that bind specifically towards the concentrate on antibody and block antibody binding. These peptide typically incorporates the epitope acknowledged because of the antibody.

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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to make sure the very best effects are...

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This information outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best results are...

Antibodies bound to the blocking peptide now not bind to the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, you can see which staining is certain.

Goal Info Cyclin E belongs to your highly conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance through the mobile click here cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a complex with and functions being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates within the G1-S period boundary and is particularly degraded as cells progress as a result of S section.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...

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